Acidipila

[A.ci.di.pi'la N.L. n. acidum (from L. adj. acidus, sour] an acid; L. fem. n. pila a ball or sphere; N.L. fem. n. Acidipila acid sphere). Cells stain Gram-negative and are nonmotile cocci and coccobacilli. Aerobic, acidophilic, and chemoorganotrophic. Good carbon sources for growth are sugars, gluconate, and some amino acids. The main components of cellular fatty acids are C15:0 iso and C16:1ω7c. Menaquinone-8 is the major quinone. The phylogenetic position is in subdivision 1 of the phylum Acidobacteria. Habitats: AMD and acidic soil. The type species is A. rosea. Acidipila rosea (ro’se.a L. fem. adj. rosea rose colored, pink). In addition to GSK1120212 nmr the characteristics shown in the description of the genus, the following are observed. Cells are cocci and coccobacilli measuring 0.5–0.8 μm in diameter. Cells are capsulated. Colonies on solid media are circular, smooth, translucent, mucous, and pink. The temperature range MS-275 cost for growth is 22–37 °C (optimum 30 °C). The pH range for growth is 3.0–6.0 (optimum pH 4.5). Usable carbon and electron donor sources are l-arabinose, d-xylose, d-fructose, d-glucose, d-galactose, d-mannose, glycerol, cellobiose, d-lactose, maltose, trehalose, gluconate, myo-inositol, sucrose, l-glutamate, histidine, casamino acids, yeast

extract, and peptone. Those not utilized are d-mannitol, d-sorbitol, methanol, ethanol, acetate, propionate, butyrate, caprylate, lactate, succinate, fumarate, malate, tartrate, benzoate, aminobutyrate, malonate, oxalate, p-hydroxybenzoate, alanine, l-aspartate, leucine, or serine. The G+C content of the DNA is 59.5 mol% (by HPLC). The type strain is strain AP8T (=NBRC 107607T=KCTC 23427T). We are grateful to Prof. Norio Wakao, Faculty of Agriculture, Iwate University, for supplying us with acid mine water samples. This work was carried

out as a part of the 21st Century COE Program ‘Ecological Engineering and Homeostatic Human Activities’ founded by the Ministry of Education, Sports, Culture, Science and Technology, Japan. The GenBank/EMBL/DDBJ accession number for the Phenylethanolamine N-methyltransferase 16S rRNA gene sequence of strain AP8T is AB561884. Fig. S1. Phase-contrast and transmission electron micrographs of cells of strain AP8T. Please note: Wiley-Blackwell is not responsible for the content or functionality of any supporting materials supplied by the authors. Any queries (other than missing material) should be directed to the corresponding author for the article. “
“In this work, we analyzed motility and the flagellar systems of the marine bacterium Vibrio shilonii. We show that this bacterium produces lateral flagella when seeded on soft agar plates at concentrations of 0.5% or 0.6%. However, at agar concentrations of 0.7%, cells become round and lose their flagella. The sodium channel blocker amiloride inhibits swimming of V.