The construction of HIV 1 like particles occurs in this method according to the modified method that originated for building virus like particles on the basis of the murine leukemia virus that is associated with HIV 1. Many laboratories involved in the search purchaseAfatinib for new anti-hiv providers don’t are able to work directly with the infectious replication competent virus. This sort of study, involving personnel getting into direct contact with the normal virus, can be executed only in licensed laboratories that provide conditions that assure operational security and have permission to deal with class III hazard infectious substances. In this regard, the development and use of safe cell systems to test anti-viral activity is of rather large importance in the design means of new therapeutic agents. Lentiviral vectors, whose practical activity manifests itself as a result of the activity of all HIV 1 enzymes, are of particular interest for secure and expeditious screening of potential inhibitors of HIV 1 replication. Since the early 1980s, vectors based on simple and complex retroviruses have now been intensively used Plastid as powerful universal resources, including those for creating effective transfer systems and for the appearance of different genes and interfering RN As in human and animal cells both in vitro and in vivo. Lentiviral vectors have already been found in our laboratory, as well as in other laboratories, as a way to design safe systems for the testing of inhibitors of wild type HIV 1 replication. These methods are represented by a recombinant lentivirus carrying a fragment of the HIV 1 genome, without the regions that encode virus peptides and retain the gene of a reporter protein. More over, pseudoviral particles are made up of the enzymes that are needed for HIV 1 replication, which supplies the potential to synthesize a DNA content with this genome, as well as the possibility to incorporate it in to the purchase Linifanib host cell genome via the same mechanism as the one at play in the contagious HIV 1. It is essential that these pseudo HIV 1 particles may carry coat proteins of HIV 1 or other enveloped viruses on their floor, depending on researchers decision. This allows the likelihood of using particular lines of eukaryotic cells and sufficiently high disease efficiency. This process consists in personal introduction of plasmids containing a) the gag pol gene of HIV 1 that encodes the structural proteins for the development of the capsid of a viral particle and HIV 1 enzymes, b) the env gene that encodes glycoproteins of the HIV 1 envelope or the gene of the envelope protein of another virus, and c) antiviral DNA that encodes the recombinant RN A genome containing the marker gene of the fluorescent protein to the cultivated human embryonic kidney cells.