However, the presence of abnormal DC precursors in the fetal and pre-diabetic pancreas of NOD mice indicates that the autoimmune process in the NOD mouse starts much earlier.

Several studies showed aberrancies already in the pre-diabetic NOD mice. An increased level of the extracellular matrix protein fibronectin was found in the early postnatal NOD pancreas, and is associated with an enhanced accumulation of macrophages and altered islet morphology 17. In the early neonatal pancreas of NOD mice abnormalities in DC and macrophage populations were described 18. ER-MP58 is a marker which is present on all myeloid progenitors. However, some non-myeloid cells can express this marker at low levels 15. Isolated ER-MP58+ cells from the pancreas were used in cultures with GM-CSF and developed into DCs. Only cells of the myeloid Ceritinib mouse lineage will respond to this growth factor 19. BM cells from NOD mice have previously been shown by several groups to have reduced responses to GM-CSF 20, 21. In contrast, myeloid precursors from NOD fetal pancreas showed an increased response to GM-CSF compared with C57BL/6. These cells had an increased proliferation and produced BMS-777607 more DCs, suggesting a proliferation and/or apoptotic defect in myeloid

precursors in the NOD fetal pancreas and indicating towards an intrinsic abnormality of these cells. Interestingly, it has been described that NOD myeloid cells have a high GM-CSF expression 22. This suggests that if the pancreatic precursors exhibit this phenotype as well, O-methylated flavonoid an autocrine loop driven by GM-CSF might contribute

to the abnormal expansion and differentiation of the local pancreas DC precursors in the NOD mouse. However, a contribution of additional signals from the pancreatic tissue itself might explain why at specific ages waves of DC accumulation have been observed. Our observations on the presence of abnormal local precursors in the NOD pancreas are suggestive for a new concept on the role of local pancreatic DC precursors in the development of diabetes. This proposed model differs from current paradigms of acute inflammation, where Ly6Chi monocytes are recruited from the circulation to a site of pre-autoimmune injury to become DCs 23–25. In our concept inflammation and organ-specific autoimmunity use different routes for accumulation of DCs in target organs-to-be and suggest that the accumulating DCs in the NOD pancreas are different from the well-characterized TNF/iNOS-producing DCs (TIP-DCs) that are recruited from the peripheral blood to sites of inflammation. A large body of research has been carried out on the development of DCs in various lymphoid tissues from BM precursors. The macrophage and DC precursor (MDP) for lymphoid tissue conventional DCs (cDCs), pDCs and monocytes is characterized as a cell expressing Lin−c-kithiCD115+CX3CR1+Flt3+ 8, 26.