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Monoclonal Antibody Targeting Sialyl-di-Lewisa– Containing Internalizing and Noninternalizing Glycoproteins with Cancer Immunotherapy Development Potential A C

Silvana T. Tivadar1, Richard S. McIntosh1, Jia Xin Chua2, Robert Moss1, Tina Parsons2, Abed M. Zaitoun3, Srinivasan Madhusudan1, Lindy G. Durrant1,2, and Mireille Vankemmelbeke2
ABSTRACT

Tumor glycans constitute attractive targets for therapeutic anti- bodies. The sialylated glycocalyx plays a prominent role in cancer progression and immune evasion. Here, we describe the charac- terization of the mAb, FG129, which targets tumor-associated sialylated glycan, and demonstrate its potential for multimodal cancer therapy. FG129, obtained through BALB/c mouse immu- nizations with liposomes containing membrane glycan extracts from the colorectal cancer cell line LS180, is an mIgG1k that targets sialyl-di-Lewisa–containing glycoproteins. FG129, as well as its chimeric human IgG1 variant, CH129, binds with nanomolar functional afﬁnity to a range of colorectal, pancreatic, and gastric cancer cell lines. FG129 targets 74% (135/182) of pancreatic, 50% (46/92) of gastric, 36% (100/281) of colorectal, 27% (89/327) of ovarian, and 21% (42/201) of non–small cell lung cancers, by IHC. In our pancreatic cancer cohort, high FG129 glyco-epitope expres-

sion was signiﬁcantly associated with poor prognosis (P 0.004). Crucially, the glyco-epitope displays limited normal tissue dis- tribution, with FG129 binding weakly to a small percentage of cells within gallbladder, ileum, liver, esophagus, pancreas, and thyroid tissues. Owing to glyco-epitope internalization, we val- idated payload delivery by CH129 through monomethyl auris- tatin E (MMAE) or maytansinoid (DM1 and DM4) conjugation. All three CH129 drug conjugates killed high-binding colorectal and pancreatic cancer cell lines with (sub)nanomolar potency, coinciding with signiﬁcant in vivo xenograft tumor control by CH129-vcMMAE. CH129, with its restricted normal tissue dis- tribution, avid tumor binding, and efﬁcient payload delivery, is a promising candidate for the treatment of sialyl-di-Lewisa– expressing solid tumors, as an antibody–drug conjugate or as an alternative cancer immunotherapy modality.

Introduction
Recent advances in cancer cell glycomics have highlighted the differential glycan make-up of tumor cells versus their normal coun- terparts. During transformation, cancer cells alter their glycosylation proﬁle because of genetic, epigenetic, and metabolic reprogramming of glycosyl transferase activity as well as the availability of nucleotide- glycan donors (1–3). This results in glycan structures with altered branching (N-glycans), truncation due to incomplete synthesis, increased sialylation and fucosylation as well as higher density gly- cosylation (O-glycans), or altogether novel structures due to neosynth- esis (4, 5). This altered glycome affects cancer cell biology, as over 50% of the cellular proteome and a signiﬁcant fraction of membrane lipids are glycosylated that affects their folding, distribution, and activity (6).

1Division of Cancer and Stem Cells, School of Medicine, City Hospital Campus, University of Nottingham, Nottingham, United Kingdom. 2Scancell Limited, University of Nottingham Biodiscovery Institute, Nottingham, United Kingdom. 3Section of Surgery, School of Medicine, Queens Medical Centre, University of Nottingham, Nottingham, United Kingdom.
Note: Supplementary data for this article are available at Molecular Cancer Therapeutics Online (http://mct.aacrjournals.org/).
Corresponding Author: Lindy G. Durrant, Scancell Limited, University of Nottingham Biodiscovery Insitute, University Park, Nottingham NG7 2RD, United Kingdom. Phone/Fax: 4411-5823-1863; E-mail: [email protected]
Mol Cancer Ther 2020;19:790–801
Speciﬁcally during cancer progression, altered glycosylation and sialylation of proteins and lipids have profound effects, ranging from immune evasion to increased cellular proliferation and the capacity to metastasize. Immune evasion stems from the interaction of tumor sialoglycans with immune inhibitory sialic acid–binding immuno- globulin-type lectins (siglecs) expressed by innate immune cells, thereby dampening immune cell activation or evading natural killer– mediated killing (7–11). Increased proliferation results from direct and indirect effects on growth factor signaling, and the interaction of sialylated glycans with activated endothelial cell selectins supports cancer cell metastasis (12–14). Oversialylation of a range of human gastrointestinal and pancreatic cancers as well as melanoma has consistently been associated with enhanced proliferative and meta- static potential (15–18).
The original carbohydrate antigen (CA)19.9 was identiﬁed via the CA19.9 mAb, obtained through immunizations with the human colon carcinoma cell line SW-1116. CA19.9 is a sialyl-Lewisa–containing monoganglioside glycolipid that is overexpressed in many gastroin- testinal and pancreatic cancers (19, 20). In pancreatic and colon cancer, CA19.9 on secreted mucins is the most consolidated carbo- hydrate tumor marker, used for early diagnosis as well as for mon- itoring responses to therapy (21–24).
Here we describe the generation of FG129, a murine (m) IgG1 with kappa light chain, which predominantly targets sialyl-di-Lewisa– containing glycoproteins. We include extensive evaluation of the FG129-binding speciﬁcity as well as its glyco-epitope distribution. On the basis of the favorable FG129 glyco-epitope distribution, we report the creation of a chimeric human (h) IgG1 version, CH129, and demonstrate its potential for payload delivery to target-expressing solid tumors.

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Materials and Methods
Materials, cells, and antibodies
All cancer cell lines—gastric (AGS and MKN45), colorectal (COLO205, HCT-15, HT29, LoVo, LS180, DLD1 and SW480), pan-
creatic (ASPC1 and BxPC3), lung (H69, DMS79 and EKVX), ovarian (OVCAR3, OVCAR4, and OVCA433), breast (DU4475 and MCF-7),
as well as the murine myeloma NS0 cell line and normal human umbilical vein endothelial cells (HUVEC)—were purchased from ATCC. All cell lines were authenticated using short tandem repeat proﬁling. The mAb CA19.9 (ab15146, RRID:AB_301691) was pur- chased from Abcam, the anti-HLA-ABC (clone W6/32) from eBioscience, and the anti-CD40 from R&D Systems. Incomplete Freund’s adjuvant was from Sigma-Aldrich and alpha- galactosylceramide from Alexis Biochemical. Human serum albumin (HSA)–conjugated sialyl-Lewisa: monosialyl, monofucosyllacto-N- tetraose-APD-HSA was obtained from IsoSepAB.

Generation of mAbs
Plasma membrane lipid extract from 5 107 LS180 cells, incor- porated into liposomes, was used to immunize BALB/c mice at two- weekly intervals over a 2-month period, with alpha-galactosylceramide and anti-CD40 mAb included as adjuvants. Five days after the ﬁnal immunization, the splenocytes were harvested and fused with NS0 myeloma cells. Hybridoma supernatant was screened by ELISA and by ﬂow cytometry for LS180 reactivity. Stable clones were established by repeated limiting dilutions and the FG129 mAb was puriﬁed from hybridoma supernatant using standard protein G afﬁnity chromatog- raphy. mAb isotyping was performed using a standard isotyping kit (Mouse Monoclonal Antibody Isotyping Test Kit, Bio-Rad).

FG129-variable regions’ cloning and generation of CH129 (hIgG1) expression construct
Total RNA was prepared from 5 106 FG129 hybridoma cells using TRIzol (Invitrogen) following the manufacturer’s protocol. First- strand cDNA was prepared from 3 mg of total RNA using a First- Strand cDNA Synthesis Kit and AMV reverse transcriptase following the manufacturer’s protocol (Roche Diagnostics). PCR and sequencing of heavy- and light-chain variable regions was performed by Syd Labs, Inc and variable region family usage analyzed using the IMGT database (25). FG129-variable regions were subsequently cloned into the hIgG1/kappa double expression vector pDCOrig-hIgG1 (26) and the sequence conﬁrmed by sequencing.

CH129 HEK293 transfection and mAb puriﬁcation
The CH129 mAb was obtained following transient transfection of Expi293F cells using the ExpiFectamine 293 Transfection kit (Gibco, Life Technologies). Brieﬂy, HEK293 cells in suspension (100 mL,
2 106/mL) were transfected with 100 ng DNA and conditioned medium harvested at day 7 posttransfection. The CH129 mAb was puriﬁed using standard protein G afﬁnity chromatography and dia- lyzed against PBS.

Indirect immunoﬂuorescence and ﬂow cytometry
Cancer cells (1 105) or whole blood (50 mL/well) was incubated with primary mAbs (at 33.3 nmol/L) for 1 hour at 4◦C, as described previously (27); followed by 1-hour incubation at 4◦C with anti-mouse FITC-labeled secondary antibody, lysis of red blood cells (for whole blood binding analysis; Cal-Lyse, Invitrogen), and ﬁxing in 0.4% formaldehyde (Sigma). Cells were run on Beckman Coulter FC-500 and analyzed using WinMDI 2.9. Determination of speciﬁc antibody

binding capacity (SABC), corresponding to the mean number of accessible antigenic sites per cell was performed using the QiFi kit (Dako) according to the manufacturer’s instructions.

Western blot analysis
Cancer cell lysates (from 1 105 cells) and total lipid extracts (from
1 106 cells) were subjected to SDS-PAGE (4%–12% Bis-Tris, NOVEX, Invitrogen) and transferred to Immobilon-FL PVDF mem- branes (EMD Millipore). Triplicate samples were loaded and mem- branes were blocked for 1 hour [5% (w/v) milk in PBS-Tween 20 (0.05% (v/v)] followed by incubation with primary mAbs (7.4 nmol/L) in 2% BSA-PBS-Tween 20 (0.05% (v/v)) overnight at 4◦C. Negative control consisted of omission of primary antibody. Secondary anti- bodies were IRDye 800CW donkey anti-mouse or IRDye 800CW goat anti-human (LI-COR Biosciences), both used at 1:5,000 for 1 hour at room temperature. mAb binding was visualized using a LI-COR Odyssey scanner.

Lewis antigen and sandwich ELISA
ELISA plates were coated with 100 ng/well Lewis-HSA antigens (direct ELISA) or 200 ng/well FG129/CH129 (for sandwich ELISA), blocked with 2% (w/v) BSA-PBS, followed by incubation with primary mAb (direct ELISA) or sera from healthy donors, patients with pancreatic cancer, or mouse serum from a COLO205 xenograft model (sandwich ELISA). Bound antibody (direct ELISA) was detected using streptavidin-HRPO (Thermo Fisher Scientiﬁc); bound antigen (sand- wich ELISA) was detected using in-house biotinylated-FG129 and streptavidin-HRPO. Plates were read at 450 nm on a Tecan Inﬁnite F50.

Glycan array analysis
FG129 was screened by the Consortium for Functional Glycomics (CFG) for binding to 600 natural and synthetic glycans (core H group, version 5.1). Brieﬂy, slides were incubated with 300 nmol/L FG129 for 1 hour, before detection with Alexa Fluor 488–conjugated secondary mAb.

Afﬁnity determination
The kinetic parameters of FG129 and CH129 binding to sialyl- Lewisa-APD-HSA (sialyl-di-Lewisa is not commercially available) were determined by surface plasmon resonance (SPR; Biacore 3000, GE Healthcare). Increasing concentrations (0.3 nmol/L–200 nmol/L) of FG129 and CH129 were injected across a sialyl-Lewisa-APD-HSA– coupled CM5 chip and binding data ﬁtted, using BIA evaluation 4.1. The chip contained four cells, two of which, HSA-coated (in-line reference cells), the other two were coated with low [30 response units (RU)] and high amounts (360 RU) of sialyl-Lewisa-APD-HSA, mim- icking cell surfaces with low and high glyco-epitope expression.

IHC
Tumor and normal tissue binding was assessed on tissue micro- arrays (TMA) as described previously (27). In brief, after antigen retrieval and blocking of endogenous peroxidase activity and nonspe- ciﬁc binding sites, the slides were incubated with FG129 (7.4 nmol/L) at room temperature for 1 hour, followed by detection with a bioti- nylated secondary mAb (Vector Laboratories). The slides were sub- sequently incubated with preformed streptavidin/biotin-HRPO (Dako Ltd.) and 3,30-diaminobenzidine and ﬁnally counterstained with hematoxylin. Staining was scored by two independent assessors, using New Viewer software 2010. The sections were given a semiquantitative histologic score (H-score, 0–300) based on the cellular staining

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intensity: negative (0), weak (1), moderate (2), and strong (3) and on the percentage of positive cells. Stratiﬁcation cut-off points for the survival analysis (SPSS 13.0, SPSS Inc) were analyzed using X-Tile software (28). P values < 0.05 were considered signiﬁcant.

Patient cohorts
The study populations included cohorts from the consecutive series of 462 archived colorectal cancer specimens (1994–2000; median follow up 42 months; censored December 2003; ref. 29); patients with lymph node–positive disease routinely received adjuvant chemother- apy with 5-ﬂurouracil/folinic acid), 350 ovarian cancer samples (1982– 1997; median follow-up 192 months; censored November 2005; patients with stage II to IV disease received standard adjuvant che- motherapy, which in later years was platinum based; ref. 30), 142 gas- tric cancer samples (2001–2006; median follow up 66 months; cen- sored Jan 2009; no chemotherapy; ref. 31), 68 pancreatic and 120 biliary/ampullary cancer samples (1993–2010; median 45 months; censored 2012; 25–46% of patients received adjuvant chemotherapy with 5-ﬂuorouracil/folinic acid and gemcitabine; ref. 32), and 220 NSCLC (January 1996–July 2006: median follow up 36 months; censored May 2013; none of the patients received chemotherapy prior to surgery but 11 patients received radiotherapy and 9 patients received at least 1 cycle of adjuvant chemotherapy postsurgery) obtained from patients undergoing elective surgical resection of a histologically proven cancer at Nottingham University Hospital (Nottingham, Unit- ed Kingdom) or Derby University Hospitals (Dery, United Kingdom). No cases were excluded, unless the relevant clinicopathologic material/ data were unavailable. Studies involving human participants were approved by the Nottingham Research Ethics Committee and were in accordance with the 1964 Helsinki declaration and its later amend- ments or comparable ethical standards. Written consent was obtained from all participants.

Competition ﬂow cytometry–based cell binding assay
AlexaFluor 488–labeled [according to the manufacturer's protocol (Invitrogen) FG129, 66.7 nmol/L] was preincubated for 1 hour at 37◦C with 100 mL of undiluted serum from patients with pancreatic cancer or healthy human volunteers. Subsequently, cell binding to preﬁxed (1% formaldehyde) HCT-15 (1 105 cells) was analyzed on a Beck- man Coulter FC-500 (WinMDI 2.9).

Confocal microscopy
AlexaFluor 488 (495/519nm)-labeled FG129 was added to 1.5 105 cancer cells on glass coverslips and incubated for 1 hour at 37◦C. During the last 30 minutes of the incubation, Hoechst 33258 (350/461 nm) nucleic acid stain (1 mg/mL), and LysoTracker deep red (647/668 nm) lysosomal stain (50 nmol/L), and CellMask Orange (554/567nm) plasma membrane stain were added to the cells (Invitrogen). Cells were imaged with a ZEISS LSM 510 ConfoCor II confocal microscope (63
1.4 NA oil objective) and LSM Image Browser was used for image processing.

mAb internalization and payload delivery assay
Payload delivery by FG129 was initially evaluated by measuring the cytotoxicity of immune-complexed FG129 with a saporin-conjugated anti-mouse or anti-human (33). Fab-ZAP secondary conjugate (Advanced Targeting Systems; ref. 33). Target cells were plated overnight in triplicate into 96-well plates (2 103 cells, 90 mL/well). After preincubation (30 minutes at ambient temperature) with a concentration range of FG129 and 50 ng of the Fab–ZAP conjugate, 10 mL of conjugate or free FG129 were added to the wells and incubated

for 72 hours. Control wells, consisted of cells incubated without conjugate, incubated with secondary Fab-ZAP without primary mAb and incubated with a control mAb preincubated with Fab-ZAP. Cell viability was measured by 3H-thymidine incorporation during the ﬁnal 24 hours. Normalized results are expressed as a percentage of cells incubated with primary mAb only (control).

Drug conjugation
The following payload and linker chemistries were used for CH129:
(i) auristatin monomethyl auristatin E (MMAE) linked via a cleavable dipeptide valine-citrulline (vc) linker, CH129-vc-MMAE, including a para-aminobenzylalcohol (PABA) self-immolative spacer; (ii) may- tansinoid emtansine, DM1, via the noncleavable linker succinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate) (SMCC); and
(iii) maytansinoid DM4 via the hindered disulphide linker succini- midyl 3-(2-pyridyldithio)propionate (SPDP) CH129-DM4, which is intermediately cleavable. Direct payload conjugation, via reduced interchain disulﬁdes, was performed by ADC Biotechnology Ltd. Brieﬂy, Tris (2-carboxy-ethyl)-phosphin-HC–reduced CH129 (for- mulated at 2.3 mg/mL in PBS) was pH-adjusted to approximately pH
7.5. The antibody was then conjugated to MMAE by the addition of ﬁve molar equivalents of vc MMAE (60 minutes). CH129 conjugation to DM1-MCC and DM4-SPDP occurred in a similar manner, at 10 molar equivalents relative to the mAb (2 hours). Conjugates were puriﬁed on a G25 desalting column. A matched set of control (non- targeting) rituximab (10 mg/mL in pH 6.5 citrate buffer)-based antibody–drug conjugate (ADC) constructs was also produced. The drug-to-antibody ratios (DAR) were determined following size- exclusion chromatography (SEC) in the case of MMAE conjugates and by hydrophobic interaction chromatography (HIC) for the DM1 and DM4 conjugates (Supplementary Table S1).

In vitro ADC cytotoxicity
The cytotoxic effect of the CH129-ADC or control RTX-ADC constructs was assessed using the water-soluble tetrazolium salt WST-8 (CCK8 kit, Sigma-Aldrich). Brieﬂy, after overnight plating of cancer cells (2 103 cells/well), the ADC constructs were added at different concentrations in a ﬁnal volume of 10 mL/well, and the plates incubated at 37◦C (5% CO2) for 72 hours. WST-8 reagent was added (10 mL/well) followed by a further 3-hour incubation after which the OD 450nm was determined (Tecan Inﬁnite F50) and the surviving fraction calculated. EC50 values were determined using nonlinear regression (GraphPad Prism v 5).

In vivo ADC tumor control in COLO205 xenograft model
The study was conducted by CrownBio UK under a UK Home Ofﬁce Licence in accordance with NCRI, LASA, and FELAS guidelines. Animal welfare for this study complies with the UK Animals Scientiﬁc Procedures Act 1986 (ASPA) in line with Directive 2010/63/EU of the European Parliament and the Council of September 22, 2010 on the protection of animals used for scientiﬁc purposes. Subcutaneous tumors of a human colorectal adenocarcinoma model of COLO205 were established in age-matched female BALB/c nude (Charles River) mice via injection of 5 106 viable cells in 0.1 mL serum-free RPMI: Matrigel (1:1) into the left ﬂank of each mouse. Mice (n 10) were randomly allocated to treatment groups based on their tumor volume (mean tumor volume per group at start of dosing: 63.2 mm3 3.9 mm3, study day 7) and dosed intravenously (i.v.), biweekly, with 0.1 mg CH129-ADC or RTX-ADC (control) or vehicle (PBS, 100 mL) for a total of four doses. Tumor growth was monitored up until week 4. Body weight and tumor volume were assessed three times weekly and

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reduction in tumor volume analyzed statistically using a two-way ANOVA test with Bonferroni posttest (interaction factors; GraphPad Prism v 7.4, GraphPad Inc).

Results
FG129 as well as chimeric hIgG1, CH129, recognize their
sialyl-di-Lewisa glyco-epitope on glycoproteins from a range of cancer cell lines
FG129 is a mouse IgG1 with kappa light chain (Supplementary Fig. S6). Variable region sequencing demonstrated that the FG129 heavy chain belongs to the mouse heavy chain IGHV10-1ω02 (IGHD1- 1ω01, IGHJ4ω01) family with three mutations compared with the parental germline sequence. The FG129 light chain belongs to the mouse kappa chain IGKV8-19ω01 (IGKJ4ω01) family with two muta- tions compared with the parental germline sequence.
Glycan array (CFG) analysis of the FG129 glyco-epitope binding proﬁle revealed a high speciﬁcity of FG129 for sialyl-di-Lewisa (100%) as well as the two closely related glycans, sialyl-Lewisa-Lewisx (89%) and sialyl-Lewisa (89%), the latter only when presented on a long carbon spacer (eight carbons, sp8; Fig. 1A; Supplementary Table S2 and http://www.functionalglycomics.org/glycomics/HServlet?operation view&sideMenu no&psId primscreen_6165). FG129 did not bind the closely related, dietary-derived, N-glycolylneuraminic acid (Neu5Gc)-Lewisa, nor did it bind unsialylated Lewisa, Lewisx, sialyl- Lewisc, or sialyl-Lewisx, indicating that its speciﬁcity is largely driven by terminal and accessible sialyl-Lewisa recognition. In addition, the recognition was speciﬁc for sialic acid in alpha 2–3 linkage as no binding to alpha 2–6 or alpha 2–8 sialylated glycoconjugates was detected.

The overall cancer cell surface binding by FG129 was analyzed using ﬂow cytometry. FG129 showed strong cell surface binding [geometric mean (Gm) 1,000] to colorectal tumor cell lines such as HCT-15 and COLO205, somewhat lower to pancreatic lines such as ASPC1 and BxPC3; moderate binding (Gm 100–1,000) to LS180, DLD1, MKN45, DMS79, and H69 and low binding (Gm < 100) to HT29. FG129 displayed negligible binding to AGS, SW480, EKVX, MCF-7, LoVo, DU4475, OVCAR3, OVCAR4, and OVCA433 (Fig. 1B; Sup-
plementary Fig. S1A). Quantitative analysis of the antibody-binding capacity (ABC) on a subset of the high- to moderate-binding cell lines revealed 6.2 × 105 antigenic sites for COLO205 compared with
2.2 × 105 for HCT-15 and approximately 1.2 × 105 for both BxPC3 and

Figure 1.

Sialylated glyco-epitope recognition on cancer cell surface glycoproteins by FG129 and CH129. A, Fine binding speciﬁcity of FG129 by high-density glycan array screening (CFG, core H, version 5.1). ‘Sp’ denotes the length of the spacer between the glycan and the slide. Inset shows the top binding glycan structures as listed in Supplementary Table S2. The complete results for FG129 have been deposited on the CFG website. B, Overview of FG129 binding (33.3 nmol/L) to a range of pancreatic, colorectal, gastric, lung, and breast cancer cell lines via indirect immunoﬂuorescence staining and ﬂow cytometric analysis. C, Comparison of cell surface binding by CH129 of a subset of cancer cell lines compared with FG129 (33.3 nmol/L) via indirect immunoﬂuorescence staining and ﬂow cytometric analysis. D, Western analysis of cell lysates and total lipid (TL) extract from selected colorectal and pancreatic cancer cell lines. i, FG129; ii, CH129; iii, CA19.9 mAb (SPM110). Lane M, molecular marker; lane 1, COLO205 cell lysates (1 105 cells); lane 2, COLO205 TL (1 106 cells); lane 3, HCT-15 cell lysates (1 105 cells); lane 4, HCT-15 TL (1 106 cells); lane 5, BxPC3 cell lysates (1 105 cells); lane 6, BxPC3 TL (1 106 cells). The 129 mAbs only bound glycoprotein species, in contrast to the CA19.9 mAb SPM110 that also recognizes sialyl-Lewisa on glycolipids, which do not resolve on SDS-PAGE and run at the dye front (arrow).

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Table 1. Determination of FG129 and CH129 functional afﬁnity for sialyl-Lewisa-APD-HSA and cancer cell surface.
SPR Real-time sialyl-Lewisa-APD-HSA bindinga,b

FACS Cell surface binding

ELISA Sialyl-Lewisa- APD-HSA binding

mAb Association
rate, kon (1/Ms)

Dissociation rate, koff (1/s)

Dissociation constant, Kd (nmol/L)

Half maximal effective concentration,
EC50 (nmol/L)
5 –4FG129 6.2 × 10 1.1 × 10 0.18 21 0.3
5 —5CH129 4.5 × 10 8.2 × 10 0.18 59 0.6
aHigh-density SPR chip.
bThe apparent binding afﬁnity for FG129 and CH129 on the low-density chip was 43 nmol/L and 21 nmol/L, respectively.

ASPC1. Crucially, FG129 did not bind granulocytes or lymphocytes from a representative normal blood donor (Supplementary Fig. S1B) nor did FG129 bind to normal HUVECs (Supplementary Fig. S1C). These results identiﬁed several high-binding colorectal as well as pancreatic cancer cell lines with which to assess FG129-based ther- apeutic modalities in vitro.

A clinically more relevant hIgG1 chimeric variant, CH129, was created via cloning of the FG129 heavy- and light-chain variable sequences in-frame with the hIgG1 constant regions. CH129 bound a similar range of cancer cell lines compared with FG129 (Fig. 1C), with strong binding observed on COLO205 and HCT-15, moderate binding to DLD1, BxPC3, ASPC1, and LS180 and low binding on DMS79.
On the cancer cell surface, glyco-epitopes can be present on glycoproteins as well as glycolipids. Consequently, we assessed rec- ognition of biologically relevant glyco-targets by FG129 and CH129, in more detail, by Western blotting using total lipid cell extracts and whole-cell lysates from colorectal (COLO205 and HCT-15) and pancreatic (BxPC3) cell lines. FG129, as well as CH129, bound to a range of predominantly high-molecular weight (50–200 kDa) glyco- proteins in all lysates, with more intense staining observed for COLO205 compared with the HCT-15 and BxPC3 lysates, potentially reﬂecting the differences in ABC (Fig. 1D). Interestingly, both the COLO205 and BxPC3 staining patterns displayed a band of very high molecular weight, not observed for the HCT-15 lysate, perhaps suggesting mucin binding. In comparison, the CA19.9 glycan as deﬁned by mAbs 19-9 and 52a is a monosialoganglioside, a sialylated glycolipid derivative of lacto-N-fucopentaose II (20, 34). Accordingly, Western blot analysis of the same cell lysates and total lipid extracts with CA19.9-recognizing SPM110 showed a prominent glycolipid band at the dye front, in all three total lipid extracts, as well as a number of glycoprotein species (Fig. 1D).

FG129 and CH129 bind their glycotarget with nanomolar functional afﬁnity, in vitro and on the cell surface
The glycome analysis indicated that the preferred glyco-target of FG129 was sialyl-di-Lewisa closely followed by sialyl-Lewisa, but only if presented on a complex sugar or long carbon spacer. As sialyl-di- Lewisa was not commercially available as a conjugate, we opted for a sialyl Lewis a–HSA conjugate with the glycan linked to HSA lysine residues via an extended acetyl phenylene diamine (APD) spacer, for performing in vitro binding studies. We thus set out to measure FG129 and CH129 functional afﬁnity using the aforementioned glycan conjugate and SPR analysis, as well as through ELISA. SPR afﬁnity measurements of FG129 and CH129 on the sialyl-Lewisa- APD-HSA–coupled CM5 chip revealed functional afﬁnity of the order of 0.1 nmol/L on the high-density surface, governed by fast on-rates

(105 Ms—1) and a slow off-rates (10—4 s—1; Table 1) and monovalent nanomolar ( 10 nmol/L) afﬁnity, on the low-density surface. In addition, concentration-dependent sialyl-Lewisa-APD-HSA antigen binding was assessed by ELISA, where FG129 as well as CH129 bound sialyl-Lewisa-APD-HSA with subnanomolar EC50 (Table 1; Supple- mentary Fig. S2A). Cancer cell surface functional afﬁnity was assessed using ﬂow cytometry and ﬁtting the results to a one-site speciﬁc binding model, after subtraction of nonspeciﬁc binding. This revealed an apparent nanomolar Kd for FG129 and CH129 on COLO205 (Supplementary Fig. S2B; Table 1) as well as HCT-15 and BxPC3 (Supplementary Fig. S2C and S2D, respectively).

FG1290s glyco-epitope distribution covers an extended range of human tumor tissues, combined with minimal normal tissue cross-reactivity
The tumor tissue binding of FG129 was assessed by IHC. FG129 bound to 74% (135/182) of pancreatic tumors, 50% (46/92) of gastric tumors, 36% (100/281) of colorectal tumors, 27% (89/327) of ovarian, and 21% (42/201) of NSCLC tumors (Table 2). Representative images of different staining levels of tumor tissues are shown in Fig. 2A. In the pancreatic cancer cohort, Kaplan–Meier analysis of disease-free sur- vival of patients with pancreatic revealed a signiﬁcantly lower mean survival time in the high FG129–binding group (high, mean survival: 30 months (n 94)) compared with the lower FG129 binding group (low, mean survival: 90 months; n 82; P 0.004, log-rank test). On multivariate analysis using Cox regression, high FG129 antigen expression in pancreatic cancer was a marker for poor prognosis which was independent of perineural invasion and lymph node involvement (P 0.012) which are key prognostic factors in pancreatic cancer. This suggests that in this cohort, patients with high-density glyco-epitope expression would beneﬁt from FG129-based therapy.
The normal tissue distribution of FG129 was evaluated using two normal human TMAs: AMSbio, T8234708-5, covering 31 tissues, one normal human individual per tissue, in duplicate, and US Biomax, FDA999i, 32 types of normal organs from three individuals, single core per case. On the AMSbio array, FG129 displayed a very restricted binding pattern and did not bind most normal tissues, including vital tissues such as heart, brain, stomach, and kidney (Supplementary Table S3; Fig. 2C). Weak to moderate binding of a very small percentage of cells was seen in gallbladder, ileum, liver, esophagus, pancreas, and thyroid. In contrast, the CA19.9 mAb stained a subset of tissues: esophagus, liver, and pancreas with strong intensity (Supple- mentary Table S3). In addition, the majority of tissues on the US Biomax array were negative with low-to-moderate staining of a small fraction of cells on tonsils (1/3), thymus (2/3); salivary gland (1/3); esophagus (3/3); adjacent normal (1/3), and cancer adjacent uterine cervix tissue (2/3; Supplementary Fig. S3).

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Table 2. Binding of FG129 (7.4 nmol/L) by IHC to gastric, colorectal, pancreatic, ovarian, and lung TMAs, by staining intensity.

Tissue

Gastric
Colorectal Pancreatic/biliary/ ampullary
Ovarian Lung (adenocarcinoma)
Staining (%) (n/total)
Negative 50 (46/92) 64 (181/281) 25 (45/180) 73 (238/327) 79 (159/201)
Weak 27 (25/92) 26 (72/281) 21 (37/180) 19 (63/327) 10 (21/201)
Moderate 11 (10/92) 9 (25/281) 34 (61/180) 6 (21/327) 4 (9/201)
Strong 12 (11/92) 1 (3/281) 21 (37/180) 2 (5/327) 6 (12/201)

FG129 recognizes secreted glyco-antigen in the serum of patients with pancreatic cancer and a mouse COLO205 xenograft model, but not healthy donors

CA19.9 can be expressed on glycolipid (monosialoganglioside) as well as mucins and detection of this antigen in sera of pancreatic and colorectal cancer is used in clinical practice to monitor disease progression and response to therapy (16, 23, 35). This is currently the only FDA-approved test for pancreatic cancer. As FG129 detects terminal/accessible sialyl-Lewisa–containing glycoproteins, we ana- lyzed a subset of patient sera from our pancreatic cancer cohort for the presence of glyco-antigen using sandwich ELISA. No secreted glyco- antigen was detected in 12 sera from healthy donors, whereas in 33% (7/21) pancreatic cancer sera, glyco-antigen levels were signiﬁcantly raised (Fig. 3A). Similarly, serum from a mouse COLO205 xenograft model, included as a positive control, also contained signiﬁcantly elevated secreted glyco-antigen (Fig. 3A). We next set out to inves- tigate whether the presence of secreted glyco-antigen would impede tumor cell binding, using ﬂow cytometry. FG129 was preincubated at 37oC with the relevant sera, after which cell binding to HCT-15 was evaluated (Fig. 3B). FG129 maintained HCT-15 binding after pre- incubation with patients’ sera, suggesting that its nanomolar cell surface functional afﬁnity is sufﬁcient to retain tumor cell targeting, even in the presence of secreted glyco-antigen.

Efﬁcient cellular internalization of FG129 as well as CH129 and delivery of indirectly conjugated payload on high-binding cancer cells
As the murine and human 129 mAbs displayed avid glycotarget binding on high-binding colorectal and pancreatic cancer cell lines, we sought to analyze their internalization potential. Confocal microscopy of Alexa Fluor 488–labeled FG129 and CH129, incubated with COLO205 cells for 60 to 90 minutes, showed very efﬁcient internal- ization of both mAbs with a signiﬁcant proportion colocalizing with lysosomal compartments, as well as a proportion remaining on the cell surface (Fig. 4A). Similar results were obtained using HCT-15 and BxPC3 (Supplementary Fig. S4A and S4B, respectively), suggesting that high-level 129 mAb binding at the cell surface drives target internalization.
Lysosomal targeting by the 129 mAbs was further validated through assessing the toxicity of saporin (“ZAP”, IT-48, ATS Bio)-conjugated anti-mouse/human Fab immune complexes containing FG129 or CH129 on high-binding cancer cell lines. Internalization of Fab- ZAP-FG129 led to a dose-dependent decrease in cell viability (EC50
1 pmol/L) on high-binding cells COLO205 and HCT-15 but surprisingly, not on BxPC3 (Fig. 4B, i). MUC1 expression by BxPC3 has been linked to chemoresistance as a result of increased expression of multidrug resistance (MDR) genes (36). The antigen-low cell line LoVo was refractory to Fab-ZAP-FG129 with a modest inhibition of viability only at the highest concentrations. Fab-ZAP preincubate with isotype control showed negligible cytotoxicity. Importantly, this was corroborated for CH129, where the saporin-conjugated CH129 displayed dose-dependent toxicity with picomolar EC50 on the high- binding COLO205 and HCT-15 (Fig. 4B, ii). Together, the results indicate efﬁcient internalization and payload delivery in high-binding cancer cell lines by FG129 and CH129. Advantageously, in addition to their efﬁcient internalization, the 129 mAbs retained potent ADCC effector activity on the high-binding COLO205 (Fig. 4C), and HCT-15 (Supplementary Fig. S4C), with EC50 values in the subnanomolar range ( 0.1 nmol/L), indicating that the glyco-proteins associated with mAb internalization are distinct from the ones targeted for effector functions and suggests multimodal development potential.

CH129–drug conjugate (ADC) shows high in vitro activity on high-binding cancer cell lines
To directly assess the ADC potential of CH129, we conjugated it to clinically validated linker/payload combinations (Fig. 5A), as described in the Materials and Methods Section (DAR; Supplementary Table S1) and compared their cytotoxicity to that of a nontargeting RTX-ADC control on the high-binding colorectal cancer cell lines COLO205 and HCT-15. In vitro cytotoxicity analysis of the CH129–ADC constructs on high-binding COLO205 showed potent toxicity of all three CH129–ADC constructs, CH129-vcE (65.8 pmol/L EC50), CH129-SMCC-DM1 (0.2 nmol/L EC50) and CH129-SPDP-
DM4 (0.1 nmol/L EC50), and 100% inhibition at the highest concen- tration tested (Fig. 5B; Supplementary Table S4). Activity of the CH129-ADC compounds on HCT-15 (Fig. 5C) was lower compared with COLO205, with nanomolar EC50 values for all three CH129-ADC constructs (Supplementary Table S4). HCT-15 was more resistant to killing by the CH129–ADC constructs, cell survival being about 30%– 40% at the highest concentration tested, possibly due to the presence of MDR1 or other ATP-dependent efﬂux systems (37). On the pancreatic line BxPC3, EC50 values were in the low nanomolar range (Fig. 5D; Supplementary Table S4). On COLO205 and BxPC3, CH129-vcE, with the peptidase-cleavable linker, outperformed the noncleavable linker- containing ADCs. The nontargeting control RTX-vcE showed no cytotoxicity over the entire concentration range tested, whereas RTX-DM1 only showed signiﬁcant activity at the highest concentra- tion (100 nmol/L). RTX-DM4 displayed more potent nonspeciﬁc toxicity (Supplementary Table S4), potentially due to its slightly higher DAR compared with the other control ADCs (Supplementary Table S1).
Cytotoxicity assays on cell mixtures containing increasing amounts of glyco-antigen–negative cells (AGS) in combination with the high- binding COLO205 were performed to assess the potential for bystand- er killing by CH129–ADC conjugates with cleavable linkers, such as the CH129-vcE and CH129-SPDP-DM4, and thus the possibility of targeting heterogeneous tumors containing cells expressing lower amounts or no glyco-target, as well as tumor stromal cells. The two

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Figure 2.

Strong differential tumor versus normal human tissue distribution via IHC of the FG129. A, Binding of FG129 (7.4 nmol/L) by IHC to pancreatic, colorectal, gastric, ovarian, and lung TMAs. Representative images of different staining levels are shown: (i) negative, (ii) weak, (iii) moderate, and (iv) strong (magniﬁcation 20). B, Kaplan–Meier analysis of disease-free survival in the pancreatic patients’ cohort after TMA staining with FG129. Cutoff for high versus low was determined by X-tile. C, Normal human tissue (AMSBIO) binding of FG129, showing limited binding in esophagus, gallbladder, ileum, liver, pancreas, and thyroid (magniﬁcation ×20).CH129-ADC constructs with cleavable linkers and thus bystander potential comprised CH129-vcE and CH129-SPDP-DM4, whereas CH129-SMCC-DM1, with its uncleavable linker, was used as a control in these experiments. A ﬁxed amount of antigen-positive COLO2025 cells was mixed with increasing amounts of antigen-negative AGS cells and cell killing by the CH129-ADC compounds analyzed at 1 nmol/L. This concentration was chosen for maximum killing of COLO205 and
at the same time absence of killing of antigen-negative AGS cells (Supplementary Fig. S5). Only CH129-DM4 maintained its COLO205 killing activity in the presence of increasing amounts of antigen- negative AGS cells, inducing over 50% killing at a ratio of 10:1 of AGS:COLO205 cells; CH129-vcE displayed intermediate bystander killing, showing a gradual decrease in killing activity with increasing amounts of AGS cells (Fig. 5E). Finally, CH129-DM1 with it 796 Mol Cancer Ther; 19(3) March 2020 MOLECULAR CANCER THERAPEUTICS
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Figure 3.

A, Sandwich ELISA using FG129 for the detection of secreted sialyl-di-Lewisa in sera from patients with pancreatic cancer (n 21), healthy human donors (n 12), as well as a mouse COLO205 xenograft model (n 3). Signiﬁcance was deduced from one-way ANOVA with Bonferroni corrections for multiple comparisons. B, Competition ﬂow cytometry–based assay showing AlexaFluor 488–labeled FG129 binding to HCT-15 cells, following preincubation with sera from patients with pancreatic cancer compared with sera from ﬁve healthy human donors. FG129 maintains cell surface binding in the presence of secreted glyco-antigen (ns, unpaired t test). ω< 0.05; ωω< 0.01; ns, not signiﬁcant.

uncleavable linker required a near homogenous COLO205 population to induce cell killing. Combined, the results suggest linker cleavability dependent bystander killing by CH129-DM4 as well as CH129-vcE.

CH129–vcE conjugate potently controls tumor growth in vivo in a COLO205 xenograft model
On the basis of the subnamolar in vitro killing potency (65.8 pmol/L EC50) as well as its intermediate bystander killing activity, we selected CH129-vCE for evaluation of in vivo tumor control in a COLO205 xenograft model. Tumors were established and dosing started on day 7. In this model, biweekly administration of four doses of CH129-vcE established a signiﬁcant reduction in tumor volume compared with the control ADC, RTX-vcE (two-way ANOVA, P < 0.0001; Fig. 6A). Impressively, 7 of 10 mice became tumor-free for the duration of the study. The compounds were well-tolerated, with no adverse effects on mean body weight (Fig. 6B).

Discussion
We have generated a mouse IgG1k anti-sialyl-di-Lewisa (Neu5Aca2-3Galb1-3(Fuca1-4)GlcNAcb1-3Galb1-3(Fuca1-4)- GlcNAcb) mAb, as well as a chimeric hIgG1 variant. The mAb only bound mono-sialyl-Lewisa if presented on a long carbon spacer suggesting a preference for accessible (terminal) sialyl-Lewisa -con- taining glycan. Importantly, no cross-reactivity with the dietary- derived Neu5Gc-Lewisa was observed. Characterization of the cell surface glycotarget of our 129 mAb suggests it to be selectively expressed on glycoproteins; predominantly, but not exclusively, on colorectal, pancreatic, and gastric cancer cell lines. In contrast, the CA19.9 targeting mAb, SPM110, bound both glycolipids and glyco- proteins upon Western analysis. This differential binding proﬁle may be the result of differing binding afﬁnities, combined with the distinctive glycan clustering patterns of glycoproteins compared with

lipid-associated glycans. In addition, the ﬁne-binding speciﬁcities of the two mAbs may contribute to their differing target binding proﬁle. In this context, SPM110 has been shown to cross-react with Neu5Gc- Lewisa (CFG, primscreen_5461). In addition, a recent comparison of a selection of CA19-9 targeting mAbs, currently used in clinical diagnostics, demonstrated varied recognition of Neu5Ac- as well as Neu5Gc-conjugated glycans (38).
Both 129 mAbs (FG129 (mIgG1) as well as CH129 (chimeric hIgG1) displayed high functional sialyl Lewisa binding afﬁnity: Kd 0.2 nmol/L and 20–50 nmol/L on high- and low-density surfaces (SPR), respectively, likely reﬂecting the target density-dependent differential binding modes (bivalent versus monovalent). ELISA experiments yielded similar EC50 values compared with SPR, whereas cell surface binding functional afﬁnity was slightly reduced, albeit still in the nanomolar range, presumably due to the more ﬂuid nature of the cell membrane and the occurrence of other biological processes such as internaliza- tion (27). The ﬁndings may also reﬂect the different target antigen utilized (sialyl-Lewisa-APD-HSA in ELISA and SPR vs. complex glycoproteins on the cell surface). Taken together the apparent nano- molar functional afﬁnity should enable robust tumor targeting (39). The 129 mAb glyco-target displayed a wide tumor tissue distribu- tion encompassing pancreatic, colorectal, and gastric cancers, as well as ovarian and NSCLC to a lesser degree. Increased sialylation is asso- ciated with enhanced proliferative and metastatic potential of both colorectal and pancreatic cancer, rendering sialyl-di-Lewisa targeting therapy an attractive option (22, 40–42). Earlier work has demon- strated the prognostic value of serum secreted sialyl-Lewisa in pan- creatic adenocarcinoma (23, 43). Our study reveals that the FG129 glyco-epitope expression in pancreatic cancer is also an inde- pendent marker for poor prognosis, suggesting that therapy based on the 129 mAb would have the highest impact on the more aggressive tumors. In addition, we found that FG129 recognized secreted sialyl- di-Lewisa in around 30% of the pancreatic patients’ sera, whereas

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Figure 4.
A, Effective cellular internalization and lysosomal targeting do not affect immune effector functions by FG129 and CH129. Z-stack confocal microscopy of Alexa Fluor 488–labeled FG129 (top) and CH129 (bottom) internalization in COLO205 cells, showing colocalization with lysosomal compartments. The plasma membrane was labeled with CellMask Orange (red), the lysosomes with LysoTracker Deep Red (purple), and the nucleus with Hoechst 33258 (blue; magniﬁcation 60). B, Lysosomal saporin delivery by FG129 (i) and CH129 (ii). Cytotoxicity of Fab-ZAP-FG129 on COLO205, HCT-15, BxPC3, and LoVo
cells was evaluated using 3H-thymidine incorporation as described in Materials and Methods (i). Cytotoxicity of Fab- ZAP-CH129 on COLO205 and HCT-15 cells (ii). In both cases, normalized results are presented as a percentage of the prolif- eration of cells treated with the primary mAb only. Error bars, mean SD from four independent experiments. C, ADCC activity with subnanomolar potency of CH129 and FG129 on COLO205.

negligible secreted target was detected in the serum from healthy individuals. This is a reduced frequency compared with the current diagnostic/prognostic CA19.9–based detection levels that frequently detect secreted antigen in 62%–68% of pancreatic cancer patients’ sera (38) and may reﬂect the different binding speciﬁcities as well as afﬁnities of the mAbs. Critically, under conditions mimicking in vivo application, the FG129 maintained binding to its cell surface glyco- target in the presence of secreted antigen, suggesting that secreted circulating glyco-antigen may not be detrimental to 129 mAb tumor targeting. Normal human tissues displayed very restricted 129 glyco- epitope expression. Vital tissues such as heart, brain, lungs, and kidneys were all negative, as well as most other nonvital tissues. Weak to moderate FG129 binding was observed to normal gallbladder, ileum, liver, esophagus, pancreas, and thyroid, but only on a small fraction of cells. This differential tissue binding proﬁle with strong tumor tissue reactivity and limited normal tissue cross-reactivity, renders the 129 mAb an attractive candidate for clinical development. FG129 exhibited very efﬁcient cellular internalization and lysosomal localization as demonstrated by two indirect approaches, confocal microscopy and targeted toxin delivery. Internalization of indirectly toxin-conjugated 129 mAbs led to killing of high-binding colorectal cancer cell lines with picomolar EC50. The internalization was directly dependentonthe cell surface antigen density incell lines, with high glyco- epitope–expressing cell lines internalizing better than low-density–expressing cell lines. On that basis, we directly conjugated CH129 with three clinically validated linkers and drugs [auristatin (vcMMAE, cleav- able) as wells as maytansinoids (SMCC-DM1, uncleavable and SPDP- DM4, moderately cleavable] thereby covering a range of linker chem- istries, as well as bystander killing potential. All three CH129-ADCs exhibited subnanomolar killing of high-binding colorectal cancer lines and nanomolar killing on moderately binding colorectal cancer as well as pancreatic cell lines. CH129-vcMMAE consistently exhibited the most potent killing against uniform cells, whereas CH129-DM4 exerted the most efﬁcient bystander killing in mixed (antigen-positive and negative cells) cultures. As such, CH129-vcMMAE exerted potent in vivo tumor control in a COLO205 xenograft model. Interestingly, our analysis indicated that the sera from mice with established tumors contained secreted glyco-antigen; however, this did not impeded the signiﬁcant in vivo activity of CH129-vcE. Alternative site-speciﬁc conjugation strategies, as well as more potent drugs, for example the very potent Pyrrolobenzodiazepine (PBD), that are currently being evaluated, would constitute attractive alternatives for conjugation to the 129 mAb (44, 45). Importantly, in addition to drug conjugate potential, the 129 mAb maintains strong ADCC effector function, suggesting that alternative approaches with cancer targeting utility could be adopted. These include T-cell redirecting bispeciﬁc (TCB) formats in combination with an anti-CD3 arm, as well as the use of the 129 variable regions for reformatting for chimeric antigen receptor (CAR)-T approaches, both

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Figure 5.

A, Potent in vitro cytotoxicity of CH129–ADC compounds on colorectal and pancreatic cell lines. Schematic drawing of the three CH129-ADC compounds used in this study. In vitro cytotoxicity of activity of CH129-ADC compounds on COLO205 (B); HCT-15 (C), and BxPC3 (D). E, Bystander killing activity of CH129-ADC compounds at 1 nmol/L in mixed cultures of antigen-positive (COLO205) and antigen-negative (AGS) cells.of which rely on minimal normal tissue cross-reactivity (46). Recently, an attractive strategy for increased tumor-speciﬁc targeting in the TCB setting was proposed in the shape of an avidity-optimized HER2 TCB (47). This strategy hinged on the combination of low monovalent target afﬁnity, precluding normal tissue (with low target expression) binding, with avidity-driven tumor targeting and this approach could be relevant for the 129 mAb.

In this global immuno-oncology era, cancer-associated sialylated glycans constitute attractive targets for cancer immunotherapy. The immunologic consequences of aberrant cancer cell surface expression of sialic acid–containing glycoconjugates range from disabling the killing mechanisms of effector cells, increasing the production of

immunosuppressive cytokines, complement regulation, as well as reducing the activation of antigen-presenting cells, many aspects of which are governed by the interaction with immune-inhibitory siglec receptors (9, 10, 13, 48–50). This was recently substantiated by data demonstrating that sialic acid blockade had a major impact on tumor immune cell composition and effector cell killing ability, creating an immune-permissive tumor microenvironment (51).
In conclusion, our sialyl-di-Lewisa-targeting 129 mAb with restrict- ed normal tissue binding combined with a wide-ranging tumor distribution, favorable functional target afﬁnity, as well as efﬁcient internalization and potential multimodal application, constitutes an attractive candidate for cancer immunotherapy.

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Figure 6.

Potent in vivo tumor control by CH129-vcE ADC in a COLO205 xenograft model. A, Signiﬁcant (P < 0.0001) in vivo tumor control by 129-vcE compared with vehicle control and compared with the nontargeting RTX-vcE in a COLO205 xenograft model (Balb/c mice). B, No signiﬁcant adverse effect was observed on mean body weight during the course of the study. Arrows indicate day of dosing. Signiﬁcance was deduced from two-way ANOVA with Bonferroni corrections for multiple comparisons.

Disclosure of Potential Conﬂicts of Interest
S.T. Tivadar reports receiving a commercial research grant from Scancell Ltd (during PhD studies, received a scholarship from Scancell Ltd covering 2 years of tuition fees and stipend). L.G. Durrant is a CSO, reports receiving a commercial research grant from, and has ownership interest (including patents) in Scancell Ltd. No potential conﬂicts of interest were disclosed by the other authors.

Authors’ Contributions
Conception and design: S.T. Tivadar, T. Parsons, L.G. Durrant, M. Vankemmelbeke Development of methodology: S.T. Tivadar, R.S. McIntosh, A.M. Zaitoun,
M. Vankemmelbeke Acquisition of data (provided animals, acquired and managed patients, provided facilities, etc.): S.T. Tivadar, R.S. McIntosh, A.M. Zaitoun, S. Madhusudan Analysis and interpretation of data (e.g., statistical analysis, biostatistics, computational analysis): S.T. Tivadar, R.S. McIntosh, A.M. Zaitoun,
L.G. Durrant, M. Vankemmelbeke
Writing, review, and/or revision of the manuscript: T. Parsons, A.M. Zaitoun,
S. Madhusudan, L.G. Durrant, M. Vankemmelbeke

Administrative, technical, or material support (i.e., reporting or organizing data, constructing databases): S.T. Tivadar, R.S. McIntosh, J.X. Chua, R. Moss,
S. Madhusudan
Study supervision: L.G. Durrant
Other (assessed in pathology): A.M. Zaitoun

Acknowledgments
We are grateful for confocal imaging assistance by Tim Self (School of Life Sciences, University of Nottingham, Nottingham, United Kingdom), as well as for Biacore access (Prof. Stephanie Allen, School of Pharmacy, University of Nottingham, Nottingham, United Kingdom). This work was supported by grants from the MRC (RA4850; to L.G. Durrant).

The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Received March 4, 2019; revised October 25, 2019; accepted December 9, 2019;
published ﬁrst December 23, 2019.

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AACRJournals.org Mol Cancer Ther; 19(3) March 2020 801

Monoclonal Antibody Targeting Sialyl-di-LewisaContaining Internalizing and Noninternalizing Glycoproteins with Cancer Immunotherapy Development Potential
Silvana T. Tivadar, Richard S. McIntosh, Jia Xin Chua, et al.
Mol Cancer Ther 2020;19:790-801. Published OnlineFirst December 23, 2019.

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