The results demonstrated that MG132 induced apoptosis was mediated by activation of p38MAPK, Bak, and mitochondria dependent caspase stream including caspase 9, 3, 7, and 8, where natural compound library tension mediated activation of caspase 12 was important for the mutual activation of caspase 9 and 3. Our results also indicated that both ER stressmediated activation of p38MAPK and caspase 12 and following mitochondrial cytochrome c release were increased in the current presence of p56lck in Jurkat T cells. The proteasome inhibitor MG132 was purchased from Sigma Chemical. An ECL Western blotting equipment was obtained from Amersham. Anticytochrome c, anti Fas, and anti FasL were purchased from Pharmingen. Anti phospho JNK, anti JNK1, anti Grp78/BiP, anti CHOP/GADD153, anti caspase 3, anti poly polymerase, anti Bax, anti p56lck, anti BclxL, anti Bcl 2, and anti w actin were purchased from Santa Cruz Biotechnology. Anti phospho p38MAPK, anti p38MAPK, anti caspase 8, anti caspase 9, anti caspase 7, anti Bad, anti Bid, anti phospho p56lck, and anti phosphop56lck were acquired from Cell Signaling Technology. Anti caspase 12 was obtained from BD Sciences, and anti BAG3 was obtained from Abcam. The wide array caspase inhibitor z VADfmk, caspase 8 inhibitor z IETD fmk, anti Bak, anti Bax, JNK inhibitor SP600125, Plastid and the Src like kinase inhibitor PP2 were obtained from Calbiochem. The caspase 9 inhibitor z LEHD fmk and the caspase 3 inhibitor zDEVD fmk were obtained from BD Sciences, and the caspase 12 inhibitor z ATAD fmk and the caspase 4 inhibitor z LEVD fmk were obtained from Biovision. The p38MAPK chemical SB202190 was bought from Biomol. Annexin V FITC apoptosis set was purchased from Clontech. Human severe leukemia Jurkat T cell line E6. 1, Jurkat T cell clone A3, and FADD bad Jurkat T cell clone I2. 1 were obtained from ATCC. Human acute leukemia Jurkat T cell clones J/ Neo and J/Bcl xL were given by Dr. Dennis Taub. p56lck Stable transfectant JCaM1. 6/lck and p56lck inferior JCaM1. 6/vector were supplied from Dr. Arthur Weiss. Jurkat T cells were maintained in RPMI 1640 containing 10 % FBS, 20 mM HEPES, 5 dhge 10_5 M b mercaptoethanol, and 100 mg/ml gentamycin. For the culture of J/Neo cells, J/Bcl xL cells, JCaM1. 6/lck, and JCaM1. 6/vector, G418 was included with RPMI ATP-competitive ALK inhibitor 1640 medium at a of 400 mg/ml. The cytotoxic aftereffect of MG132 on Jurkat T cells was examined by MTT assay. Fleetingly, cells were put into the serial dilution of MG132 in 96 well plates. At 20 h after incubation, 50 ml of MTT solution was put into each well and incubated for an additional 4 h. After centrifugation, the supernatant was removed from each well, and then 150 ml of DMSO was included with dissolve the colored formazan crystal created from MTT. OD values of the answers were measured at 540 nm with a plate reader.