Lytic CD4 T cell clones may control replication of HIV and SIV in both CD4 T cells and macrophages. Numerous cytokine release by lymphoid cells is associated with superior control of HIV 1 replication, T-cell suppressor activity, and longterm non progression to AIDS. In mice immunized with IN gene variations, all IL 2 positive CD8 T cells stimulated with TNF a, 0 and IN peptides ubiquitin lysine produced IFN c. 2% of CD8 T cells co expressed IFN c, IL 2 and TNF an and hence belonged to the polyfunctional Tc1 phenotype. The vast majority of CD4 T cells also co expressed sometimes two or all three cytokines and thus belonged to the polyfunctional Tc1 phenotype. Co expression of TNF an and IFN c indicated why these IN specific CD4 T cells were the effectors operating through TRAIL mediated apoptosis,, while co release of IFN c, TNF an and IL 2 recognized the population of effector CD4 T cells able to perforin mediated target cell-killing. The cytotoxic and perforin cytokines/ TRAIL based killing take into account the majority of lytic actions of CD4 T cells. Immunization with IN gene versions was apparently able to induce at least one of the effector mechanisms. Urogenital pelvic malignancy More over, IN gene immunization produced integrasespecific antibodies which recognized both agreement FSU An integrase and a clade T integrase with similar end-point titers. Thus, IN gene versions could produce antibodies against epitopes typical for integrases of clade An and B. Eventually, we evaluated the ability of the anti IN immune reaction to eliminate the transfected expressing cells in the immunization sites. This is done by assessing the degree of expression in the injection websites of the reporter gene of firefly luciferase, co delivered together with the IN gene variants. Co injection of Luc reporter gene having a potent gene immunogen results in an immediate reduction of the in vivo reporter activity, once we have recently shown. Here, company supply of IN and Luc genes led to a significant, 10 to 15 fold decrease in the total photon mapk inhibitor flux in the site of immunization three days post immunization. We found inverse correlations of luminescence with IFN c/TNF an and IFN c/IL 2/TNF an expression by CD8 and with dual IFN c/IL 2 and double IFN c/IL 2/TNF an expression by CD4 T cells. Correlations of luminescence with IFN c/TNF a production by CD4, and with IFN c/IL 2 production by CD8 T cells didn’t reach the level of significance indicating that to affect the luminescence, CD4 T cells observed on IL 2, and CD8 T cells, on TNF a, each featuring the respective effector T cells. This supported the concept of luminescent fading being as a result of T cell mediated clearance of the expressing cells from immunization sites. Further, this indicates the role in clearance of immunogen/reporter expressing cells of the lytic CD4 Th1 cells.