\n\nAims: To compare the efficacy and safety of sublingual versus vaginal misoprostol for mid-trimester pregnancy termination.\n\nMethods: We conducted a meta-analysis of published randomised controlled trials that compared sublingual and vaginal routes. Primary outcome measures were complete abortion rate at 24 and 48 h and induction-abortion interval, and the secondary outcome measures were side effects and patients’ preference for the route. Pooled risk ratios were calculated for categorical variables, and continuous variables were compared by means of weighted

mean differences.\n\nResults: Both routes’ efficacy was similar following 24 h of treatment (pooled RR 1.04, 95% CI 0.93-1.7). Successful induction percentage after 24 h was significantly higher in nulliparous women with vaginal misoprostol (pooled RR 0.78; 95% CI 0.71-0.87). The efficacy after 48 h was significantly greater with vaginal misoprostol in the general population Elafibranor datasheet (pooled RR 0.96; 95% CI 0.93-0.99) and in nulliparous women (pooled RR 0.89; 95% CI 0.86-0.95). The sublingual route shortened

the induction-fetal expulsion PLX4032 purchase interval (WMD -4.54, 95% CI -8.03 to -1.05) and was the route preferred among women. No statistically significant differences between treatment groups were observed for placental retention or for any side effect except for fever, which was more common in the vaginal group.\n\nConclusions: Sublingual and vaginal misoprostol are safe and effective for mid-trimester pregnancy termination. The differences obtained between both

routes probably do not have clinical consequences.”
“The diversity of Cryptosporidium at species, subtype family and subtype level in diarrhoeic children was investigated in four provinces in South Africa. A total of 442 stool samples from children <5years of age were collected under a large rotavirus surveillance programme and analysed by ZiehlNeelsen acid-fast staining. Fifty-four (12.2%) were positive for Cryptosporidium, of which 25 were genotyped by polymerase chain reaction (PCR)restriction fragment length polymorphism (RFLP) and DNA sequence Nutlin-3 solubility dmso analyses of the 18S rRNA gene. The majority of genotyped specimens were identified as C.hominis (76%), and a high genetic diversity was found with five different C.hominis subtype families (Ia, Ib, Id, Ie and If). Cryptosporidium parvum was found in 20% of the isolates, and three subtype families were identified (IIc, IIe and IIb), with subtype family IIc being the most common. One specimen was identified as C.meleagridis of the subtype family IIId. These results are in accordance with findings from other developing countries and report for the first time the presence in South Africa of C.meleagridis, various subtypes of C.parvum and the subtype family Ie of C.hominis. The results suggest that C.hominis and anthroponotic C.parvum subtypes are the major cause of cryptosporidiosis in South Africa.