Coupled with a rich surface chemistry for further functionalization and excellent conductivity, NPG has great potential for applications in heterogeneous catalysis, electrocatalysis, fuel cell technologies, and biomolecular sensing in comparison
with other mesoporous materials [10–13]. In our previous work, enzyme-NPG biocomposites were successfully constructed by assembling various enzymes (such as lipase, catalase, and horseradish peroxidase) onto NPG . Among these enzymes, lipase has gained particular interest FK228 nmr as one of the most frequently used biocatalysts in the hydrolysis and the synthesis of esters from glycerol and long-chain fatty acids . In SN-38 supplier addition, lipase is commercially important and has many applications in food industry and clinical analysis . Especially, lipases are important drug targets or marker enzymes in the medical field. Recently, the development of lipase sensors has been strongly focused on biosensors for
the detection of triglycerides and cholesterol . Therefore, further studies were carried out on the catalytic performance Sapitinib supplier of the lipase-NPG biocomposite in this study. It is revealed that the pore size of NPG and adsorption time play significant roles in enzyme loading, leaching, activity, and reusability. The finding should be useful for the creation of biocatalysts and biosensors. Methods 4-Nitrophenyl palmitate, p-nitrophenol, pyrogallol, and lipase (Aldrich 534641 from Pseudomonas
cepacia) were purchased from Sigma-Aldrich (St. Louis, MO, USA). NPG was made by chemically dealloying Cepharanthine AgAu alloy foils (Ag78Au22 at.%, 25 μm in thickness, purchased from Changshu Noble Metal Company, China) in concentrated HNO3 (approximately 67%). NPG with a pore size of 35 nm was obtained by chemically dealloying AgAu alloy foils in concentrated HNO3 (approximately 67%) at 30°C for 2 h. The preparation of NPG with a pore size of 100 nm was that AgAu alloy foils was chemically dealloyed in concentrated HNO3 (approximately 67%) at 30°C for 2 h and then annealed at 250°C for 10 min. After rinsing in distilled water, the samples were dried and kept in a desiccator for further use. The morphology of the samples was observed with a JSM-6700 F field emission scanning electron microscope (SEM; JEOL Ltd.