Nat Genet 2001, 28: 53–57.CrossRefPubMed 33. Cha MY, Kim CM, Park YM, Ryu WS: Hepatitis B virus X protein is essential for the activation of Wnt/beta-catenin signaling in hepatoma cells. Hepatology 2004, 39: 1683–1693.CrossRefPubMed 34. Ding Q, Xia W, Liu JC, Yang JY, Lee DF, Xia J, Bartholomeusz G, Li Y, Pan Y, Li Z, et al.: Erk associates with and primes GSK-3beta for its inactivation resulting in upregulation of beta-catenin. Mol Cell 2005, 19: 159–170.CrossRefPubMed 35. Shtutman M, Zhurinsky J, Simcha I, Albanese C, Amico M, Pestell R, Ben Z, ev A: The cyclin D1 gene MLN8237 in vitro is a target of the beta-catenin/LEF-1 pathway. Proc Natl Acad Sci USA 1999, 96: 5522–5527.CrossRefPubMed

36. Tetsu O, McCormick F: Beta-catenin regulates expression of cyclin D1 in colon carcinoma cells. Nature 1999, 398: 422–426.CrossRefPubMed 37. Kawate S, Fukusato T, Ohwada S, Watanuki A, Morishita Y: Amplification of c-myc in hepatocellular carcinoma: correlation with clinicopathologic features, proliferative activity and p53 overexpression. Oncology 1999, 57: 157–163.CrossRefPubMed Competing interests selleck chemicals llc The authors SIS3 ic50 declare that they have no competing interests. Authors’ contributions XT carried out molecular studies, collected and analyzed the data, performed the statistical analysis and drafted the manuscript. JL carried out IHC studies.

MZM and CZ carried out part of real-time PCR studies. WDF collected the samples and participated in the design of the study. YMW designed the concept of this study and approved the final manuscript. All authors read and approved the final manuscript.”
“Background Nowadays breast cancer is becoming Montelukast Sodium the second leading cause of cancer deaths in females, almost 10% women have the

risk of developing breast cancer [1]. Although great improvements have been made in curing breast cancer, the overall five-year survival rate remains < 50% and many patients relapse after surgical resection because of the dispersion of undetectable cancer cells [2, 3]. Therefore, it is necessary to establish sensitive and specific techniques for the detection of occult tumor cells. A better method for early diagnosis may help in predicting recurrence and planning appropriate therapies to improve survival [4, 5]. Many investigations have indicated that epithelial cells from the initial tumor can be recognized in peripheral blood or bone marrow aspirates of patients with breast cancer [6, 7]. The detection of circulating tumor cells (CTCs) in the peripheral blood of cancer patients has been associated with recurrence and metastasis of breast cancer [8–10]. Cytokeratins (CKs), characteristic intermediate filament of epithelial cells, especially CK19, are widely used to detect tumor cells derived from epithelial tissues [11, 12].