Recent drug development programs focus not only on increased novel anti microtubule drugs, but in addition on novel mitotic drug targets that include mitotic kinesins and mitotic kinases MAPK activity. In addition, the usage of the cell cycle arrest that is abrogated by drugs imposed by DNA damaging agents leading to an entry into mitosis in the presence of DNA damage is just a promising technique to stimulate mitosis associated cell death in cancer cells. This review summarizes the newest progress of anti mitotic drugs and the analysis of novel antimitotic drug targets. Microtubules, together with actin and intermediate filaments are the major the different parts of the cytoskeleton of eukaryotic cells. In interphase and differentiated cells, microtubules form fibers that serve as tracks for the intracellular transport of vesicles and organelles. When cells enter mitosis, this interphase system is reorganized and dissolved in to a mitotic spindle that’s required for the congression of chromosomes and the following segregation of sister chromatids. Microtubules are round, hard and long tubes with a length of approximately 25 nm composed of _ and _ tubulin heterodimers that polymerize into 13 protofilaments, which form the wall of the microtubule. Microtubules are very dynamic structures that shrink and constantly grow, a procedure termed dynamic instability. This powerful polymerization behavior is influenced by the hydrolysis Urogenital pelvic malignancy of GTP, which can be bound at tubulin subunits. The 2 ends of microtubules are distinct: one end, the plus end, is a great deal more dynamic and can show a net growth while the other, the minus end, is anchored in the centrosome or microtubule organizing heart, and is less dynamic and can show net shrinkage. Ergo, at certain time, microtubules can show no change within their fat size, however display quite high dynamics. The second significant dynamic behavior of microtubules is termed treadmilling, which is a healthy natural compound library net shortening at the minus end and a net expansion at the plus end. Consequently, treadmilling results in a movement of tubulin subunits from the plus to the minus end of microtubules. Equally, dynamic instability and treadmilling are very important features for the big event of microtubules, especially all through mitosis. Many different proteins can bind to microtubules. Some of them are structural proteins, called microtubule associated proteins that control the security and the dynamic behavior of microtubules. The other large group of microtubule connected proteins is represented by motor proteins, which is often grouped in to kinesins and dyneins. Several of those proteins shift along microtubules mediating intracellular freight transportation, others have certain functions in mitosis in centrosome location, chromosome congression and segregation.