Results Single exposure with sevoflurane anesthesia in young WT mice increased the levels of P GSK3B and P AKT in brain tissues http://www.selleckchem.com/products/Roscovitine.html of the mice Activation of AKTGSK3B signaling pathway, demon strated as increases in the levels of P GSK3B and P AKT, has been reported to protect cellular toxicity. We therefore set out to study the effects of sevoflurane anesthesia on the levels of P GSK3B and P AKT. P GSK3B immunoblotting showed that there was a visible increase in the levels of P GSK3B in brain tissues of the mice treated with sevoflurane as compared to that of the mice treated with the con trol condition. There was no significant difference in the B Actin levels in the brain tissues of the mice treated with sevoflurane as compared Inhibitors,Modulators,Libraries to that of the mice treated with the control condition.

Inhibitors,Modulators,Libraries Quantification of the Western blot, based on the ratio of P GSK3B levels to B Actin levels, showed that the sevoflurane anesthesia in creased the P GSK3B levels as compared to the control condition 182% versus 100%, P 0. 005. Next, we assessed the effects of the sevoflurane anesthesia on P AKT levels in the brain Inhibitors,Modulators,Libraries tissues of mice. P AKT immunoblotting showed that there was a visible increase in the levels of P AKT in brain tissues of the mice treated with sevo flurane as compared to that of the mice treated with the control condition. There was no significant difference in the B Actin levels between the sevoflurane anesthesia and the control condition. Quantification of the Western blot, based on the ratio of P AKT levels to B Actin levels, showed that the sevoflurane anesthesia increased the P AKT levels as com pared to Inhibitors,Modulators,Libraries control condition 153% versus 100%.

Multiple exposures with sevoflurane anesthesia in young WT mice decreased the levels of P GSK3B and P AKT in the brain tissues of the mice Our previous studies have shown that anesthesia with 3% sevoflurane two hours daily for one day in P6 does not induce cognitive impairment in the mice at P30. However, the anesthesia with Inhibitors,Modulators,Libraries 3% sevoflurane two hours daily for three days is able to induce cognitive impairment in the mice at P30. Given that single sevoflurane anesthesia increased the levels of P GSK3B and P AKT, next, we assessed the effects of multiple exposures with sevoflurane anesthesia on the levels of P GSK3B and P AKT in the brain tissues of young mice. P GSK3B immunoblotting demonstrated a visible decrease in the levels of P GSK3B in brain tissues of the mice treated with the sevoflurane anesthesia as compared to that of the mice treated with control condition. There selleck inhibitor was no significant difference in the B Actin levels be tween the sevoflurane anesthesia and the control condi tion.