The animals underwent surgical exposure of the neck; the right common carotid artery was used as the test and the left common carotid artery as the control. On the 21st day after surgery, animals were sacrificed for removal of the arteries, measurements, and histological analysis. We determine formation
of aneurysm to occur when the test artery dilated compared to the control.
Results There was no aneurysm formation in the sham group. The papain group showed aneurysm formation in all cases (100%). The average diameter of the aneurysms was 3.8 +/- 1.4 mm and the average length was 16.7 +/- 6.0 mm. The histological analysis showed a destruction of the elastic fibers in 100% of cases, mild Veliparib inflammation in 62.5%, intimal fibrosis in 50%, endothelial injury in 100%, and thrombosis in 100% of cases.
Conclusion Papain was capable of forming aneurysms with histological
characteristics similar to those of elastase-induced aneurysms; however, a comparative study is necessary to determine whether the papain is superior to elastase in the production of experimental saccular E7080 mw aneurysms.”
“Aims:
To immobilize Methylobacterium sp. NP3 and Acinetobacter sp. PK1 to silica and determine the ability of the immobilized bacteria to degrade high concentrations of phenol.
Methods and Results:
The phenol degradation activity of suspended and immobilized Methylobacterium sp. NP3 and Acinetobacter sp. PK1 bacteria was investigated in batch experiments with various concentrations of phenol. The bacterial cells were immobilized by attachment to or encapsulation in silica. The encapsulated bacteria had the highest phenol degradation rate, especially at initial phenol concentrations between BAY 63-2521 mouse 7500 and
10 000 mg l-1. Additionally, the immobilized cells could continuously degrade phenol for up to 55 days.
Conclusions:
The encapsulation of a mixed culture of Methylobacterium sp. NP3 and Acinetobacter sp. PK1 is an effective and easy technique that can be used to improve bacterial stability and phenol degradation.
Significance and Impact of the Study:
Wastewater from various industries contains high concentrations of phenol, which can cause wastewater treatment failure. Silica-immobilized bacteria could be applied in bioreactors to initially remove the phenol, thereby preventing phenol shock loads to the wastewater treatment system.”
“Introduction Geometric indices of cerebral aneurysms have been widely studied to determine rupture risk. However, most of these parameters were evaluated based on two-dimensional (2D) images and could have a measurement bias. We propose a new three-dimensional geometric index, an aneurysm volume-to-neck area ratio (VNR). To determine whether the VNR of ruptured aneurysms is different from that of unruptured aneurysms, we compared VNR with other 2D geometric indices in discriminative capacity for aneurysm rupture status.