Yavuz Beyazit M.D.*, Murat Kekilli M.D.*, Tugrul Purnak M.D.†, Mevlut Kurt M.D.*, * Department of Gastroenterology, Turkiye Yuksek Ihtisas 20s Proteasome activity Hospital, Ankara, Turkey, † Department of Gastroenterology, Ankara Numune Education and Research Hospital, Ankara, Turkey. “
“The human hepatitis B virus (HBV) causes acute and chronic infections in humans and

chimpanzees. HBV infects its hosts at minimal inoculation doses and replicates exclusively in hepatocytes. The viral determinants for the pronounced species specificity and the high efficacy to address hepatocytes in vivo are unknown. Previous findings showed that N-terminally myristoylated peptides constituting a receptor binding domain of the HBV large envelope (L)-protein block HBV entry in vitro and in vivo. Here we investigate the ability of such peptidic receptor ligands to target the liver. Injection of radioactively labeled HBVpreS-lipopeptides resulted in rapid accumulation in livers of mice, rats, and dogs but not cynomolgus

monkeys. Without lipid moiety the peptide was excreted by renal filtration, indicating its possible retention through the lipid by serum factors. Organ distribution studies of 26 HBVpreS peptide variants revealed a correlation of HBV infection inhibition activity and the ability to target mouse livers. Together with complementary studies using primary hepatocytes of different species, we hypothesize that HBV hepatotropism is mediated through specific binding of the myristoylated N-terminal preS1-domain of the HBV L-protein to a hepatocyte specific receptor. Moreover, the restricted infectivity of HBV to human primates is selleck not generally determined click here by the absence of this binding receptor in nonsusceptible hosts

(e.g., mice) but related to postbinding step(s) (e.g., membrane fusion). Conclusion: HBVpreS-lipopeptides target to the liver. This observation has important clinical implications regarding the pharmacokinetic properties of Myrcludex B, the first entry inhibitor for HBV/HDV. In addition, this provides the basis for the application of the peptides as vehicles for hepatocyte-specific drug targeting. (HEPATOLOGY 2013) See Editorial on Page 9 DMSO, dimethylsulfoxide; ge, genome equivalent; HBsAg, hepatitis B surface antigen; HBV, hepatitis B virus; HDV, hepatitis delta virus; L-protein, hepatitis B virus large surface protein; PHH, primary human hepatocytes; PTH, primary Tupaia hepatocytes; p.i., postinfection; RP-HPLC, reversed-phase high-performance liquid chromatography; SPECT/CT, single photon emission computed tomography/computed tomography. The human hepatitis B virus (HBV) causes acute and chronic liver infections. Worldwide, 350 million people are persistently infected.1 Chronic HBV will remain a major global health problem, despite the availability of vaccines. Therapies (interferon-alpha [IFNα] and five nucleoside analogs) are limited and mostly noncurative.