An additional gel was run and incubated over night in TBST containing 1% BSA and one hundred thousand normal goat serum rather than the Bax main antisera, then incubated with the secondary antisera and visualised as above to manage for non specific binding. 7. Data of power of N 20 and P 19 Bax staining in the granule cell layer of control, HD and AD brains. Values represent mean standard error, ssignificantly not the same as control, p 0. 05. Only 1 HD case was analyzed for P 19 Bax.. the hippocampus, striatum and cortex, in-the granule although not Purkinje cells of the cerebellum, and in the glial cells in white matter tracts e. g. in the untreated rat head Fig. 1.. This pattern of staining was exactly like that seen in the control part of the HI rat brain. Deborah 20 Bax was also expressed in the olfactory bulb and thalamus, and in oligodendrocytes. Within the hippocampus, the granule cells and the CA2 pyramidal cells displayed the highest level of Bax immunoreactive staining, with CA3 and CA1 pyramidal cells slightly less powerful. Pre absorption with the N 20 Bax peptide completely abolished this immunoreactivity Fig. 2.. Induseum griseum and the subicular area also Organism showed strong staining. In the hippocampus of the get a handle on rat brain the staining with the G 19 Bax antiserum was much like N 20 staining Fig. 3., except that staining was significantly weaker in CA2, CA3 and the hilar region, and there was an alternative distribution of staining within the granule cells. The PC66 Bax antiserum only appeared to stain microcapilliaries, without cell staining Fig. 3.. Equally antisera showed a dramatic decline of Bax in the CA1 about the stroke side 48 h 72 biomedical library h after HI. Staining with the PC66 antiserum showed a significant p 0. 05. 3 and 4.. The remaining cells of this type were strongly showing Bax as found with the PC66 antisera compared with no staining on the control side, while there is an enormous cell loss in CA1 on the swing side 72 h after HI. In contrast, there was no change in Bax staining with any one of the antisera utilized in the granule cell layer, which doesn’t endure apoptosis fol lowing HI, on the swing side compared with the get a grip on side Fig. 4..